Fluorescence spectroscopy is a powerful analytical technique that studies the fluorescence emitted by molecules upon excitation by light. It is widely employed in chemistry, biochemistry, and materials science for its sensitivity and selectivity. The process involves exposing a sample to light of a specific wavelength, known as the excitation wavelength, which leads to the promotion of electrons to higher energy states. As these electrons return to their original states, they emit fluorescence at longer wavelengths. By analyzing the emitted light, researchers can gain valuable information about the composition, structure, and environment of the molecules under investigation. Fluorescence spectroscopy finds applications in fields such as molecular biology, environmental monitoring, and pharmaceutical research for its ability to provide detailed insights into the properties of fluorescent compounds.
